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MessagePosté le: Jeu 13 Juil - 14:42 (2017)    Sujet du message: A Primer Of Genome Science Torrent Répondre en citant

A Primer Of Genome Science Torrent >

International Congress Series 1293: 127–13051None of the rest of the fermentation parameters showed significant correlation with the diversity indexes although correlation values showed little differences between both datasets (Table 4).Table 4Spearson's correlation (rho value) between Richness (Ri) and Shannon-Wiener (Sh) indexes of NGS (OTU matrix from Ion Torrent data) or T-RFLP (peaks in the amplicons of 16S rRNA gene digested using HhaI, HaeIII, MspI and RsaI) and fermentation parameters .Canonical Correspondence Analysis between TRF's or OTU's and fermentation products is shown in Figures 4a and 4b(2011) Impact of subacute ruminal acidosis (SARA) adaptation and recovery on the density and diversity of bacteria in the rumen of dairy cowsDNA was then extracted using QIAGEN QIAamp DNA stool mini kits (Qiagen Ltd., UK) as previously described [26].Fingerprinting analysis (T-RFLP) PCR was performed using a 16S rRNA bacterial-specific primer pair, cyanine-labelled 27F (5′-AGA GTT TGA TCC TGG CTG AG-3′) and unlabelled 1389R (5′-AGG GGG GGT GTG TAG AAG-3′) [27] following Skivanov et alAppl Environ Microbiol 70: 4800–4806 [PMC free article] [PubMed]52In our study, presence of only holotrich protozoa did not alter the relative abundance of these N utilizing bacteria(2011) Metagenomic discovery of biomass-degrading genes and genomes from cow rumenOur findings suggest that classic fingerprinting methods are still valuable tools to study microbial diversity and structure in complex environments but that NGS techniques now provide cost effect alternatives that provide a far greater level of information on the individual members of the microbial populationHyperlinks from the text

Fingerprinting techniques might thus still represent an acceptable and low-cost way to study microbial diversity in complex ecosystemsor its licensors or contributorsRumen protozoa are not essential to rumen function but can significantly affect ruminal fermentation and their host's nutrition [18], so the progressive colonization of rumen protozoa served as a powerful model to evaluate changes in the rumen microbiome of sheepWhitehead R, Cooke G, Chapman B (1967) Problems associated with the continuous monitoring of ammoniacal nitrogen in river water(2010) Changes in the bacterial population of the caecum and stomach of the rabbit in response to addition of dietary caprylic acidScienceDirect is a registered trademark of Elsevier B.V.This method is widely used and provides rapid taxonomic classifications from domain to genus of both partial and full-length rRNA gene sequences

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